長片段基因合成
長片段基因合成服務以極具競爭力的價格可為您合成長達6kb以上的長片段基因,合成序列無任何突變或錯誤,保證序列100%準確。公司憑借獨有的克隆技術以及全新的長片段組裝克隆平臺支撐,可準確有效地將多個DNA片段進行一步組裝。相較于傳統的多個短片段基因多次拼接及組裝的方法,該技術大大節省了時間及成本,且所合成的序列100%準確,沒有突變及錯誤的發生。


服務訂購

基因長度

交貨周期

價格(元/bp)

備  注

6000~10000 bp

30~35 工作日

1.5


10000~15000 bp

商  議

請詢價


15000~30000 bp

商  議

請詢價


30000~50000 bp

商  議

請詢價


服務流程
服務訂購
約5ug質粒DNA凍干粉
1支含有重組質粒的穿刺菌/甘油菌
測序峰圖(abi文件電子版)
COA文件(電子版)
基因序列比對文件(電子版)

Peer-reviewed Citations:
Combined SNPs and miRNAs technologies for beef traceability
http://onlinelibrary.wiley.com/doi/10.1111/jfs.12360/full
Q Wu, G Zhou, S Yang, X Xu, C Li - Journal of Food Safety ... Premix Ex Taq Kits (Takara Biotechnology Co. Ltd., Dalian, China). Forward primers used for RT-PCR were synthesized by General Biosystems (General Biosystems Co. Ktd., Anhui, China). The forward primer sequences of the...

Assessment of the fusion tags on increasing soluble production of the active TEV protease variant and other target proteins in E. coli
https://link.springer.com/article/10.1007/s12010-016-2360-9
Xuelian Yu, Jiaqi Sun, Weiyu Wang, Li Jiang, Beijiu Cheng, Jun Fan-Applied Biochemistry and Biotechnology ...Primary and secondary antibodies as well as reagents for Western blotting were provided by Sangon Biotech (Shanghai, China). Primer synthesis and DNA sequencing were conducted by General Biosystems (Chuzhou, China)...

Engineering thermostable (R)-selective amine transaminase from Aspergillus terreus through in silico design employing B-factor and folding free energy calculations
http://www.sciencedirect.com/science/article/pii/S0006291X1632191X
J Huang, DF Xie, Y Feng - Biochemical and Biophysical Research Communications ...The AT-ATA cDNA sequence containing the NcoI and XhoI restriction sites was synthesized at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and the pET28a(+) expression vector(pET28a(+)-AT-ATA) was constructed as a template for site-directed mutagenesis...

A Vector Based on the Chicken Hypersensitive Site 4 Insulator Element Replicates Episomally in Mammalian Cells
http://www.ingentaconnect.com/content/ben/cgt/2016/00000016/00000006/art00006
Zhang,Xi;Zhang,Xi;Wang,Xiao-Yin;Wang,Xiao-Yin;Jia,Yan-Long;Jia,Yan-Long;Guo,Xiao;Guo, Xiao;Wang,Yan-Fang;Wang,Yan-Fang;Wang,Tian-Yun;Wang,Tian-Yun-Current Gene Therapy ...trans- gene expression. MATERIALS AND METHODS Vector Construction The cHS4 DNA sequence used in this study was artificially synthesized by General Biosystems, Inc. (Chuzhou, China) [23]. The synthesized sequences...

TTF1, in the Form of Nanoparticles, Inhibits Angiogenesis, Cell Migration and Cell Invasion In Vitro and In Vivo in Human Hepatoma through STAT3 Regulation
http://www.mdpi.com/1420-3049/21/11/1507/htm
Bin Xiao, Dongjing Lin, Xuan Zhang, Meilan Zhang and Xuewu Zhang-Molecules …pcDNA3.1 plasmid of STAT3 was synthesized by General Biosystems (An Hui, General Biosystems, Inc., Chuzhou, China)...

Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells
http://www.nature.com/articles/srep42805
Chun-Peng Zhao, Xiao Guo, Si-Jia Chen, Chang-Zheng Li, Yun Yang, Jun-He Zhang, Shao-Nan Chen, Yan-Long Jia & Tian-Yun Wang-Sci Rep. …a 2073-bp fragment (position 94–2166) was synthesized by General Biosystems (Chuzhou, China)…

Genome-wide analysis of Dof family genes and their expression during bud dormancy in peach (Prunus persica)
http://www.sciencedirect.com/science/article/pii/S0304423816305738
M Chen, X Liu, L Huan, M Sun, L Liu, X Chen, D Gao, L Li-Scientia Horticulturae …All of the primerswere designed using the Beacon Designer 7 software and synthesized by GeneralBiosystems (Anhui, China)…

Improving thermostability of (R)-selective amine transaminase from Aspergillus terreus through introduction of disulfide bonds
http://onlinelibrary.wiley.com/doi/10.1002/bab.1572/full
DF Xie, H Fang, JQ Mei, JY Gong, HP Wang, XY Shen, J Huang, LH Mei-Biotechnology and Applied Science … Materials The AT-ATA cDNA from A. terreus sequence, including the NcoI and XhoI restriction sites, was synthesised at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and plasmid pET28a(+) was used for gene cloning and DNA sequencing...

Tobacco etch virus protease mediating cleavage of the cellulose-binding module tagged colored proteins immobilized on the regenerated amorphous cellulose
https://link.springer.com/article/10.1007/s00449-017-1772-4
Xuelian YuJiaqi SunWeiyu WangLi JiangRuyue WangWenjun XiaoBeijiu ChengJun Fan-Bioprocess and Biosystems Engineering ... Materials and methods. Materials. Reagents used in plasmid construction and protein expression were bought from Takara (Dalian, China). Gene and primer syntheses, DNA sequencing are conducted by General Biosystems (Chuzhou, China)...
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